• The primary determinant of the target specificity of an

  2018-11-08

  

  The primary determinant of the target specificity of an individual designer nuclease is the length of its DNA recognition site, which is different in ZFNs, TALENs and the CRISPR/Cas9 system. Also, the GC content substantially influences the target specificity. In theory, a 16bp recognition site shou

• br Conclusion Cellular reprogramming is an inefficient proce

  2018-11-08

  

  Conclusion Cellular reprogramming is an inefficient process and obtaining the rare reprogramming intermediates is a major hurdle in understanding this important event at the molecular level. The application of certain techniques that require higher cell numbers such as transcription factor ginger

• In our previous studies we successfully synthesized poly

  2018-11-08

  

  In our previous studies, we successfully synthesized poly(hydroxyethyl methacrylate) (pHEMA) derived-polymers differing in the amount of 2-methacryloyloxyethyltrimethyl ammonium chloride (METAC) (Rosso et al., 2003; De Rosa et al., 2004). We demonstrated that positively charged moieties on the polym

• The karyotype of the BIOT EHMT

  2018-11-08

  

  The karyotype of the BIOT-0708-EHMT1 iPSC line was determined with Giemsa-banding, proving normal diploid 46, XX karyotype, without any detectable abnormalities (Fig. 1B). In parallel, the pathogenic NM_024757.4(EHMT1):c.3413G>A mutation was confirmed by Sanger sequencing in the newly established iP

• Also critical to the success of

  2018-11-08

  

  Also critical to the success of hard tissue engineering strategies is the selection and use of an appropriate growth factor regimen. Although several growth factors are reported to play an important role in the process of matrix mineralisation, IGF-1 is the most abundant growth factor in bone matrix

• Using the combination of a mitogen activated protein

  2018-11-08

  

  Using the combination of a mitogen-activated protein kinase kinase (MEK) inhibitor, PD0325901 (PD03), and a glycogen synthase kinase 3 (GSK3) inhibitor, CHIR99021 (CHIR), which will hereafter be referred to as 2i, mouse ESCs (mESCs) are maintained in a ground state (Ying et al., 2008). CHIR activate

• neuron specific enolase Introduction Cell to cell competitio

  2018-11-08

  

  Introduction Cell-to-cell competition among male germline stem cells has been proposed to account for the higher frequency of some disorders among children of older fathers, a correlation referred to as the paternal age effect (PAE) (Goriely and Wilkie, 2012). In addition to compelling observations

• Proper functioning of the RPE is important for maintaining t

  2018-11-08

  

  Proper functioning of the RPE is important for maintaining the health and integrity of the outer retina, photoreceptors, and choriocapillaris. Healthy RPE cells play many crucial roles in the retina, including transportation of nutrients such as glucose or vitamin A from blood to the photoreceptors,

• The activity of CXCL through CXCR receptor has

  2018-11-08

  

  The activity of CXCL12(5-67) through CXCR3 receptor has been shown before (Denoyer et al., 2012; Zhu et al., 2009). Here we aimed to confirm this finding using CXCL9, a natural ligand of CXCR3. NSCs pre-treated with CXCL9 were not susceptible to the CXCL12(5-67) apoptotic effects. The effect of CXCL

• However the use of an NHP model is

  2018-11-08

  

  However, the use of an NHP model is hampered by the limited availability of clinically relevant NHP-iPSC lines. While the majority of NHP-iPSCs described in the literature were generated using retroviral vectors, human iPSCs intended for eventual therapeutic use need to be generated using transgene-

• Zscan is sometimes considered as a marker of

  2018-11-08

  

  Zscan4 is sometimes considered as a marker of highly pluripotent status, merely due to its expression at the two-cell stage during development (Cerulo et al., 2014). However, based on our observation, we would like to propose that the expression of Zscan4 is activated in response to telomere shorten

• The current paper investigates the association between conte

  2018-11-07

  

  The current paper investigates the association between contextual state-level generalized trust and individual 2009 A(H1N1) pandemic immunization in the American states. Some prior studies have investigated the association between social capital/generalized trust and immunization. To start with Ronn

• Nonetheless the analyses presented here

  2018-11-07

  

  Nonetheless, the analyses presented here have two serious limitations. First, we provide no insight into why the imprisonment–mortality association is different for Hispanic females than for other groups (although we do hypothesize briefly above). Second, and as has long been documented in critiques

• Sleep reduction for days produces a decrease in the performa

  2018-11-07

  

  Sleep order l-ascorbic acid for 5 days produces a decrease in the performance of verbal working memory tasks [19], while other studies did not find differences [18,38]. To our knowledge there are no studies documenting the effects of sleep reduction on visuospatial memory tasks. Method Discus

• Introducci n La econom a de la informaci n

  2018-11-07

  

  Introducción La economía de la información aborda los tópicos sobre los modelos de búsqueda, información imperfecta, selección adversa, modelos de señalamiento, modelos de revelación así como el diseño de mecanismos (Kreps, 1995 y Gould y Lazear, 2002) y fue reconocida como campo de conocimiento en

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